Nowadays the technological tools, especially computers, were designed to facilitate the lives of people. In education area, these tools were available to educators and students. Technological tools, correct understanding of abstract concepts in science education have great importance. This study was carried on elementary pre-service science teachers, and perceptions of elementary pre-service science teachers were investigated about computer-supported instruction (CSI) material in chemistry instruction. Some of chemistry subjects in first and second grade of elementary education for computer-supported instruction (CSI) as a course material were prepared and it was presented to pre-service teachers. A questionnaire was developed for determination of pre-service science teachers’ opinions on computer-supported chemistry instruction and was applied to 100 pre-service teachers, who watch computer-supported instruction. The questionnaire results were analyzed using SPSS 13.0 packet program.